Reparto Principal
Uma Thurman
La Novia / Beatrix Kiddo (imágenes de archivo)
Lucy Liu
O-Ren Ishii (Cottonmouth)
Vivica A. Fox
Vernita Green (Copperhead)
Michael Madsen
Budd (Sidewinder)
Daryl Hannah
Elle Driver (California Mountain Snake)
David Carradine
Bill (Snake Charmer)
Julie Dreyfus
Sofie Fatale
Chiaki Kuriyama
Gogo Yubari
Shin'ichi Chiba
Hattori Hanzo
Chia-Hui Liu
Johnny Mo (como Gordon Liu)
Michael Parks
Earl McGraw / Esteban Vihaio
Michael Bowen
Buck
Jun Kunimura
Jefe Tanaka
Kenji Ôba
Hombre Calvo (Tienda de Sushi)
Yuki Kazamatsuri
Propietaria
James Parks
Edgar McGraw
Sakichi Sato
Charlie Brown
Jonathan Loughran
Camionero